A team of scientists from the University of Pretoria (UP) has published the only study from Africa,
that entailed genomic One Health investigations which reveal that Delta variants of COVID-19 were
transmitted from humans to animals.
One Health is an approach that recognises that the health of people is closely connected to the health of
animals and a shared environment. Close contact with animals and their environment provides opportunities
for diseases to spread between animals and people.
The team of transdisciplinary scientists found that reverse zoonotic transmission of COVID-19 from
asymptomatic animal handlers at a private zoo in Gauteng, posed a risk to big cats kept in captivity.
Transmission of the Delta variant to these animals could result in more severe disease. The animals tested PCR
positive for up to seven weeks after becoming sick. “This extended period of potential virus shedding poses a
risk of infection to animals in close proximity and possibly humans. The animals were therefore placed in
quarantine until they tested negative,” the scientists said.
The study, titled “SARS-CoV-2 Reverse Zoonoses to Pumas and Lions, South Africa, was led by Professor
Marietjie Venter, Head of the Zoonotic, Arbo- and Respiratory Virus Programme at UP’s Department of
Medical Virology; and Professor Katja Koeppel, Associate Professor of Wildlife Health at the University’s
Faculty of Veterinary Science. The findings of the study were recently published in Viruses journal.
Postdoctoral students, Dr Adriano Mendes and Dr Amy Strydom of the Zoonotic, Arbo- and Respiratory Virus
Programme were responsible for the initial diagnoses and genomic investigations of the human and animal
cases; while Dr Lia Rotherham and Professor Misheck Mulumba, Director of the Agricultural Research Council
Onderstepoort Veterinary Research Campus, confirmed the diagnoses.
“In 2020, the team tested the faeces of two pumas that had previously shown signs of anorexia, diarrhoea and
nasal discharge,” Prof Koeppel explained. “They tested positive for COVID-19, and exhibited mild symptoms.
They were medicated and made a full recovery after 23 days. Unfortunately, we could not carry out an
investigation into the source or the specific variant involved in the outbreak. The samples were diagnosed by
real-time PCR at the time of the outbreak, but when we tried to sequence the sample a year later, there was
insufficient RNA left for genome sequencing.”
A year later, during South Africa’s third wave of the COVID-19 pandemic, the team conducted a study on three
sick lions at the same zoo. “The lions had breathing problems as well as runny noses and a dry cough for up to
15 days,” Prof Koeppel said. “A persistent cough was seen between five and 15 days, with two lions
experiencing difficulty breathing. One lioness developed pneumonia that did not respond to antibiotics.”
Respiratory swabs were submitted to Prof Venter’s programme, where the cases were investigated.
Staff and lions were monitored in the weeks that followed for the presence of SARS-CoV-2, and within 15 to
25 days, all three lions made a full recovery. “A One Health investigation into the source of infection was
conducted on 12 staff members who had been in direct or indirect contact with the lions,” Prof Venter said.
“Swab and serum testing were carried out.”
One staff member who had direct contact with the lions and another who had indirect contact tested PCR
positive for SARS-CoV-2 two weeks after the start of the lion disease course. All three lions were PCR positive.
“These two individuals and three other staff members also tested positive for anti-spike IgG antibodies,” Prof
Venter said. “None of the staff interviewed reported any recent symptoms of COVID-19; however, the zoo
keeper developed mild symptoms the following day and was confirmed positive.”
More tests were done. “This data suggests that SARS-CoV-2 was circulating among staff during the time that
the lions got sick, and suggests that those with direct contact with the animals were likely responsible for the
reverse zoonotic transmission.”
Genome sequencing was conducted on the humans and three lions, and tests revealed that each of the
infections was a Delta variant. The two pumas and three lions presented with respiratory illness that was
similar to COVID-19 in humans. The animals did not respond to antibiotic treatment but recovered after
treatment with anti-inflammatory drugs and supportive care.
“Detection of viral RNA in the upper respiratory tract and the faeces, coupled with the fact that the pumas
and lions had symptoms, reveal that this virus is able to infect these animals via a natural infection route,”
Prof Venter said.
These outbreaks are at least the third and fourth of its kind in which SARS-CoV-2 has been shown to transmit
between humans and captive large felines, “although the current study is the only one that reports on genomic
One Health investigations of Delta variants transmitted from humans to animals, and the only one reported
so far in Africa,” Prof Venter added.
The scientists said the timeline of infections of the lions from a COVID-positive human is difficult to estimate
as all staff members were asymptomatic during the outbreak. They said that reverse zoonotic transmission of
SARS-CoV-2 from asymptomatic animal handlers pose a risk to large felines kept in captivity.
The scientists stress that precautions such as vaccinating staff, wearing masks when entering cages and
preparing food, infection control through use of disinfectants, and distance barriers for members of the public
should be put in place at zoos.
“This is to protect potentially endangered species from getting infected and dying,” Prof Venter and Prof
Koeppel said. “These measures are also important because of the risk of new variants emerging if the virus
establishes itself in other animal reservoirs; these variants could be transmitted back to humans.” Members
of the public are also urged to be aware of the possibility of infecting their pet cats and dogs if they have
COVID-19.
“A One Health approach – where human and animal health specialists as well as environmental specialists
work together – is essential to investigate and prevent zoonotic disease transmission,” the scientists said.
